electroporation相关论文
Intracellular delivery of functional molecules,such as DNA probes and plasmids,is an important method for investigating ......
Dengue fever,caused by dengue viruses (DENVs),is a widespread mosquito-borne zoonotic disease;however,there is no availa......
Erythropoietin gene transfer into rat testes by in vivo electropo-ration may reduce the risk of germ
Aim: To investigate the effects of rat Erythropoietin (Epo) on spermatogenesis by transferring rat Epo gene into cryptor......
Protection of the liver against CCl4-induced injury by intramuscular electrotransfer of a kallistati
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Conveniently and rapidly labeling of viral ribonucleoprotein complexes in influenza A virus with qua
Influenza A viruses(IAV)are known as a potential risk to public health,1 studying the mechanisms of IAV infecting their ......
Low-voltage Electroporation Mediated Transcutaneous Targeted Delivery of Liposomes with Entrapped An
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Enhancement of the immunogenicity of an alphavirus repIiCOn—baSed DNA vaccine against classical swin
Alphavirus replicon-based DNA vaccines have emerged as a promising approach to generation ofantigen—specific immune res......
The Method of Attaining high transfection efficiency of plasmid into neural stem cells by electropor
Objective:To optimize the conditions of transfecting eukaryotic expression vector pEGFP-N1 into neural stem cells(NSCs)f......
Guidance cues are critical for axon navigation and ultimately for the wiring of the neuro-circuits during nervous system......
Purpose The electroporation of mouse retinal ganglion cells (RGCs) is not efficient after birth,as transfection of the d......
This study presents the effects of alternating current electric field (10KHz,2000V/cm) on the fluorescence labeling of m......
Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fi
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Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bl
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Construction of novel high efficient chloroplast transformation system for microalga Phaeodactylum t
Background: Chloroplasts are ideal subcellular hosts for expression of transgenes and have been used for production of d......
以2岁奶牛输卵管为材料，用胰酶消化法分离得到了牛输卵管上皮细胞。输卵管上皮细胞在DMEM／F12培养基中生长良好。用一代牛输卵管上皮......
采用pcDNA3.0-GHRH(1-32)质粒,对断奶仔猪肌肉单次注射后电穿孔处理,观察对猪生产性能的影响。采用碱变性法提取pcDNA3.0-GHRH(1-3......
【目的】明确基于电穿孔的基因功能分析方法在家蚕Bombyx mori活体内的应用实效。【方法】针对调控家蚕幼虫体表斑纹黑色素合成的......
目的 建立能够稳定表达绿色荧光蛋白(GFP)的小鼠胚胎干细胞(ESC),并诱导其向神经细胞分化,为临床移植治疗神经系统疾病提供方便追......
目的 利用毕赤酵母诱导表达T4溶菌酶蛋白并测定其抗菌活性.方法 T4溶菌酶(T4 lysozyme)基因以N端融合的方式被准确插入到质粒pPIC9......
目的 以小鼠背根神经节(dorsal root ganglion, DRG)细胞为工具,绿色荧光蛋白为报告基因来优化电穿孔转染条件.方法 分别用传统从......
BACKGROUND In the first studies of electrochemotherapy(ECT),small cutaneous metastases were treated and only mild or mod......
目的研究阴道毛滴虫病毒(Trichomonas vaginalis virus,TVV)介导外源基因进入阴道毛滴虫体内表达的能力,探索TVV作为双链RNA病毒转......
Water disinfection is a critical step in water and wastewater treatment.The most widely used chlorination suffers from t......
Aim:To investigate the specific effects of Ca2+ on transgene expression during electroporation-mediated gene transfer in......
,Introduction of Foreign Genes into Silkworm Eggs by Electroporation and Its Application in Transgen
Electroporation as a methodology to introduce foreign genes into silkworm eggs was systematically analyzed. The foreign ......
,Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopu
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Study of the selective effect on cells induced by nanosecond pulsed electric field with the resistor
本文通过对荣华二采区10...
Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fi
Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA conta......
Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bl
Background So far, there is no efficient treatment for pulmonary fibrosis. The objective of this study was to determine ......
Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increas
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311 (Cyanophyceae) by electropor
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Genetic Transformation of Nannochloropsis oculata with a Bacterial Phleomycin Resistance Gene as Dom
The gene ble from Streptoalloteichus hindustanus is widely used as a selective antibiotic marker.It can control the phle......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
目的 探索以绿色荧光蛋白(GFP)为报告基因的重组质粒pEGFP/SDF-1a/54/KDEL电穿孔悬浮培养的Molt-4细胞的高效转染条件.方法 通过控......
目的 将戊型肝炎病毒ORF3基因转染HeLa细胞,建立能稳定表达ORF3蛋白(pORF3)的细胞株.方法 将ORF3基因片段克隆至真核表达质粒pcDNA......
陡脉冲不可逆性电击穿是一种新的肿瘤物理治疗方法.由于其独特的作用机制,不仅能有效杀灭肿瘤,且具有无热效应、操作简单、治疗时......
OPTIMIZATION OF ELECTROPORATION PARAMETERS FOR TRANSFECTION OF PLASMID DNA INTO MURINE BONE MARROW-D
Objective To explore the optimal electroporation parameters for transfection of plasmid DNA into murine bone marrow-deri......
Study of the selective effect on cells induced by nanosecond pulsed electric field with the resistor
A resistor-capacitor (RC) circuit model is proposed to study the effect of nanosecond pulsed electric field on cells acc......
目的 分离培养大鼠胎脑神经干细胞(NSC),研究电穿孔技术在NSC中的转染效率.方法 从sD胎鼠脑组织中分离、培养和扩增NSC,利用免疫荧......
10-23脱氧核酶(10-23 DNAzyme,10-23DZ)是近年来利用体外分子进化技术筛选得到的一种具有催化功能的单链DNA分子,能高效、特异催化......
目的 探索电穿孔介导的基因治疗对下颌骨DO过程中牵引间隙新生骨密度与强度的影响,从而为促进下颌骨DO新骨生成,缩短牵引周期,减少......
目的 探索电穿孔介导的基因治疗对兔下颌骨牵引成骨过程中细胞周期调节蛋白表达的影响。方法 45只新西兰大白兔，双侧下颌骨截骨后3d......
目的 研究不同剂量结核病DNA疫苗电转染后的免疫原性.方法 40只BALB/c小鼠通过随机数字表法分为8组,每组5只.其中4组分别用100 μl......
幼胚的遗传转化对研究植物胚胎发育相关基因的表达与调控具有重要意义, 也为植物遗传改良提供新的技术.本研究借助一种自制的特殊......
目的 探讨非病毒载体电穿孔(electroporation,EP)对大鼠早期骨性关节炎的基因治疗效果.方法 选用8周龄健康级雄性SD大鼠,通过切断......
香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源.但近年来,香蕉生产受到严重的病虫危害.大多数香蕉栽培品种是三倍体,......
应用弱正弦波电磁场改变细胞膜的穿透性并引起其失活和坏死.研究中,将人类癌细胞U-937和K-562放置于强度为10 mT和39 mT(50 Hz)的......
Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (Eps) inducing cell membrane pored (elect......
